Experimental approaches & methods frequently used in this lab

We examine the effects of chemokines and viral proteins using different methods including traditional biochemical/molecular biology techniques and novel imaging and proteomics approaches, as briefly described below. Primary cultures of rat and human central neurons are used as a model for the study of chemokine receptors naturally expressed by neurons. Alternative models include neuronal human cell lines expressing specific chemokine receptors. In addition, small animal models and post-mortem brain tissue samples from control and HIV-infected individuals are used for the analyses of specific pathways of interest in vivo/ex vivo.

Primary Brain Cultures
  Rat neuronal/glial cultures
    Cortical, hippocampal, cerebellar cultures
Human brain cultures
    Cortical neurons and glia
   
In vivo/Ex vivo
  Human brain tissue (IHC/LCM)
Animal models of drug abuse (morphine pellets/injections)
MOR k/o and other transgenic mouse lines
   
Live Cells Calcium Imaging
   
Microscopy
  Confocal microscopy
Multispectral Imaging (MSI)
Laser Capture Microdissection (LCM)
   
Cellular & Molecular biology/pharmacology
  RT-PCR/qPCR
Western Blot
ELISA
Transfection (electroporation and other methods)
RNAi

Links

www.nntc.org

www.ndriresources.org

www.sciencellonline.com

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