| Chemical Biology of Secondary Metabolism


Recent publications


Mechanistic crosslinking in the classroom

Journal of Chemical Education article by Kara Finzel! Published in JCE.


New probes for crosslinking

Lou Charkoudian's lab shows a nice new way to crosslink carrier proteins and partner proteins. Published in Biochemistry.


Rivas, M.A., Courouble, V.C., Baker, M.C., Cookmeyer, D.L., Fiore, K.E., Frost, A.J., Godbe, K.N., Jordan, M.R., Krasnow, E.N., Mollo, A. and Ridings, S.T., 2018. The effect of divalent cations on the thermostability of type II polyketide synthase acyl carrier proteins. AIChE Journal, 64(12), pp.4308-4318.


Rivas, M., Courouble, V., 2016 Haverford College Biochemistry Superlab, Sisto, A., Beld, J., Kokona, B. and Charkoudian, L.K., 2018. Thermal Stability of Type II Polyketide Acyl Carrier Proteins. The FASEB Journal, 32(1_supplement), pp.792-14.



•   Ma, A.T.*, Beld, J. and Brahamsha, B.,An Amoebal Grazer of Cyanobacteria Requires Cobalamin Produced by Heterotrophic Bacteria; Applied and Environmental Microbiology, 2017 *corresponding author **spotlighted


•   Finzel, K., Beld, J., Burkart, M.D. and Charkoudian, L.,Utilizing Mechanistic Cross-Linking Technology To Study Protein–Protein Interactions: An Experiment Designed for an Undergraduate Biochemistry Lab; Journal of Chemical Education, 2017


•   Thiele, G.A.C.,Friedman, C.P., Tsai, K.J.S., Beld, J., Londergan, C.H. and Charkoudian, L..,Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase–Carrier Protein Cross-Link; Biochemistry, 2017




•   Sonnenschein, E.C., Pu, Y., Beld, J. and Burkart, M.D., Phosphopantetheinylation in the green microalgae Chlamydomonas reinhardtii; Journal of Applied Phycology, online (2016)


•   Beld, J., Abbriano, R., Hildebrand, M. and Burkart, M.D., Probing fatty acid biosynthesis using chemistry, in bacteria, cyanobacteria, green microalgae and diatoms; Molecular Biosystems, 10.1039/C5MB00804B (2016)


•   Uranga, C.C., Beld, J., Burkart, M.D., Mrse, A., Ghassemian, M.,  Córdova Guerrero, I. and Hernández Martínez, R. Fatty acid ester production in the trunk disease fungus Lasiodiplodia theobromae; Biochemical and Biophysical Research Communications, accepted online (2016)


•   Tallorin, L.T., Finzel, K., Beld, J., La Clair, J.J. and Burkart, M.D., Trapping of the Enoyl-Acyl Carrier Protein Reductase• Acyl Carrier Protein Interaction, Journal of the American Chemical Society, accepted online (2016)



•   Cahill, J. F., Darlington, T. K., Fizgerald, C., Shoepp, N., Beld, J., Burkart, M. D. and Prather, K. A., Editors' Highlight Previous Article Next Article Articles ASAP Online Analysis of Single Cyanobacteria and Algae Cells under Nitrogen-Limited Conditions Using Aerosol Time-of-Flight Mass Spectrometry; Analytical Chemistry, 2015, ASAP

•   Paz-Yepes, J., Beld, J., Burkart, M. D. and Palenik, B., Grazing on marine Synechococcus by new marine heterotrophic nanoflagellate isolates, 2015,submitted

•   Ferreira-Camargo, L., Tran, M., Beld, J., Burkart, M.D. and Mayfield, S. P., Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae; AMB Express, 2015, online

•   Beld, J., Lee, D. J. and Burkart, M.D., Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering, Molecular Biosystems, 2015


•   Beld, J., Cang, H., Burkart, M.D., Visualizing the chain-flipping mechanism in fatty acid biosynthesis, Angewandte Chemie, 2014

•   Beld, J., Finzel, K. and Burkart, M.D., Versatility of acyl-ACP synthetases Chemistry & Biology, 2014, highlighted in preview

till 2014

•   Beld, J., Sonnenschein, E.C., Vickery, R.V., Noel, J.P. and Burkart, M.D., The Phosphopantetheinyl Transferases: Catalysis of a Posttranslational Modification Crucial for Life, Natural Products Reports, 31, 61-108 (2014)
•   Bruegger, J., Caldara, G., Beld, J., Burkart, M.D. and Tsai, S.-C. Polyketide Synthase: Sequence, structure, function in Natural Products: Discourse, Diversity and Design, ISBN: 978-1-118-29806-0 (2014)
•   Bruegger, J., Haushalter, R., Vagstad, A., Beld, J., Shakya, G., Mih, N., Townsend, C.A., Burkart, M.D. and Tsai, S.-C. Probing the Selectivity and Protein-Protein Interactions of a Nonreducing Fungal Polyketide Synthase Using Mechanism-Based Crosslinkers. Chemistry & Biology 20, 1135-1146 (2013) see corrigendum
•   Beld, J., Blatti, J.L., Behnke, C., Mendez, M., and Burkart, M.D. Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions, Journal of Applied Phycology, 1-11 (2013)
•   Blatti, J.L., Beld, J., Behnke, C., Mendez, M., Mayfield, S.P. and Burkart, M.D., Manipulating fatty acid biosynthesis in microalgae through protein-protein interactions, PLoS One, 7, e42949 (2012)
•   Metanis, N., Foletti, C., Beld, J. and Hilvert, D. Rescue of kinetically trapped intermediates in oxidative protein folding by selenoglutathione, Israel Journal of Chemistry, 51, 953-959 (2011)
•   Metanis, N., Beld, J. and Hilvert, D. The chemistry of selenocysteine. Patai’s chemistry of functional groups, edited by Z. Rappoport (2011)
•   Beld, J. Woycechowsky, K. J., and Hilvert, D. Diselenides as universal oxidative folding catalysts of diverse proteins, Journal of Biotechnology, 150, 481-489 (2010)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Small molecule diselenides catalyze oxidative protein folding in vivo, ACS Chemical Biology, 5, 177-182 (2009) cover art: “In contrast to disulfides, small amounts of exogenously added diselenide restore motility, and cells swim to the edge of soft agar plates.”
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Diselenide resins for oxidative protein folding, patent application EP09013216 (2009)
•   Coquiere, D., Bos, J., Beld, J. and Roelfes, G., Enantioselective artifical metalloenzymes based on a bPP Scaffold, Angewandte Chemie International Edition, 121, 6894-6897 (2009)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Catalysis of oxidative protein folding by small-molecule diselenides, Biochemistry 47, 6985-6987 (2008)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Selenocysteine as a probe of oxidative protein folding, Oxidative folding of Proteins and Peptides, edited by J. Buchner and L. Moroder, Royal Society of Chemistry (2008)
•   Steinmann, D., Nauser, T., Beld, J., Tanner, M., Günther, D., Bounds, P. L., Koppenol, W. H. Kinetics of tyrosyl radical reduction by selenocysteine, Biochemistry 47, 9602-9607 (2008)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Selenoglutathione: Efficient oxidative protein folding by a diselenide, Biochemistry 46, 5382-5390 (2007)
•   Timmerman, P., Beld, J., Puijk, W. C., and Meloen, R. H. Rapid and quantitative cyclization of multiple peptide loops onto synthetic scaffolds for structural mimicry of protein surfaces, ChemBioChem 6, 821-824 (2005)
•   Basabe-Desmonts, L., Beld, J., Zimmerman, R. S., Hernando, J., Mela, P., García Parajo, M. F., Van Hulst, N. F., Van Den Berg, A., Reinhoudt, D. N., and Crego-Calama, M. A simple approach to sensor discovery and fabrication on self-assembled monolayers on glass, J. Am. Chem. Soc. 126, 7293-7299 (2004)
•   Timmerman, P., Beld, J., Meloen, R. H., and Puijk, W. C. Method for selecting a candidate drug compound, International patent WO-2004-077062 (2004)