Olympus IX81 - Wide-field microscope

Zeiss AxioObserver Z1 – Wide-field microscope

The Zeiss AxioObserver is an inverted microscope - objectives are below the stage. This microscope is ideal for 2D or 3D imaging of thin specimens (one-cell layer).

Light Sources

a contrast image of the specimen is obtained under white light illumination
excitation and emission filters are available for imaging fluorescent probes
Excitation filters (nm) Emission filters (nm) Corresponding fluorescent probes

Objectives

(Magnification / Numerical aperture)

  • 10x/0.3 Dry (Zeiss)
  • 20x/0.4 Dry and 20x/0.8 Dry (Zeiss)
  • 63x/1.4 Oil (Zeiss)
  • 100x/1.4 Oil (Zeiss)

Detector

Hamamatsu Orca-R2 (CCD camera)

Acquisition software

Slidebook 6 with 2D and 3D deconvolution module

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