Olympus IX81 - Wide-field microscope

Olympus IX81 - Wide-field microscope

The Olympus IX81 is a fully automated inverted microscope - objectives are below the stage. This microscope is ideal for 2D or 3D imaging of fixed and live specimens. Excitation and emission filter wheels enable fast time-lapse imaging. The microscope is equipped with a temperature-controlled chamber for imaging living cells.

A stereology application is available on the IX81. Stereological techniques are performed to estimate the total number, volume, and surface area of objects in a biological structure. For instance, stereology uses random and unbiased sampling of tissue sections to extrapolate the total number of cells in a 3D structure.

A color camera is also available for histochemical and immunohistochemical staining.

Light Sources

a contrast image of the specimen is obtained under white light illumination
excitation and emission filters are available for imaging different fluorescent probes
Excitation filters (nm) Emission filters (nm) Corresponding fluorescent probes

Objectives

(Magnification / Numerical aperture)

  • 20x/0.7 Dry (Olympus)
  • 60x/1.4 Oil (Olympus)
  • 100x/1.4 Oil (Olympus)

Detectors

Hamamatsu Orca-Flash4.0 (sCMOS camera)

Lumenera Infinity 1-3C (color CMOS camera)

Acquisition software

Slidebook 6 with 2D and 3D deconvolution module, and stereology module

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