Martin Bootman

Jim Eberwine

Simon Giszler

Phil Haydon

Haruo Kasai

Ernst Niggli
 

Ernst Niggli

University of Bern, Switzerland

The Niggli Lab are pioneers in the use of 1- and 2-photon uncaging with confocal imaging to answer fundamental question in cardiac EC coupling. They were the first to perform 2-photon uncaging of calcium inside living cells. We have had a long-standing collaboration on application of our new calcium cages for 2-photon photolysis in heart muscle, and so recently we have tested our new caged Ca with the Niggli group:

Uncaging of MNI-glutamate elicited at the frog ankle from two light pulses separated by 2.65s. (RMS force is shown, raw force was recorded with an ATI 3/10 six axis transducer). These are the first examples of in vivo caging in mammals.


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