Jim Eberwine

University of Pennsylvania, Philadelphia, USA

The Eberwine Lab are pioneers in the development of technology to study mRNA in single cells. They have used these techniques to show that proteins are translated locally in dendritic compartments. We are collaborating in the use of caged mRNA to induce photochemically production of GFP-tagged proteins. Recently we have been awarded a Conte Center P20 grant together.

Uncaging of GFP mRNA in Hippocampal Neurons. Caged GFP mRNA was electroporated into these rat hippocampal neurons (A). The mRNA was uncaged in the cell soma (B). The confocal image shows production of GFP after photolysis.